師資
個人簡介：
龍艷萍,，南方科技大學(xué)研究副教授。2007年本科畢業(yè)于西北農(nóng)林科技大學(xué),，2015年博士畢業(yè)于中國科學(xué)院遺傳與發(fā)育生物學(xué)研究所,，2016年加入南方科技大學(xué)生物系翟繼先課題組從事博士后研究并工作至今,。2022年植物生物學(xué)女科學(xué)家學(xué)術(shù)交流會“優(yōu)秀女青年獎”獲獎人。現(xiàn)主要方向?yàn)榛诟咄繙y序技術(shù)的植物單細(xì)胞測序和三代長測序技術(shù)的開發(fā)和應(yīng)用以及基因表達(dá)調(diào)控機(jī)制相關(guān)研究,。主導(dǎo)開發(fā)植物單細(xì)胞核全長測序技術(shù)FlsnRNA-seq、單分子水平全長新生RNA分析方法FLEP-seq以及用于擬南芥基因組多位點(diǎn)相互作用及關(guān)聯(lián)甲基化修飾的Pore-C 技術(shù)。目前已以第一作者（含共同）和共同通訊作者在Nature Plants,、Nature protocols、PNAS,、Genome Biology,、Plant Biotechnology Journal和New Phytologist等國際主流學(xué)術(shù)期刊上發(fā)表多篇高水平研究論文。
研究領(lǐng)域：
1. 植物單細(xì)胞測序
2. 三代長測序技術(shù)的開發(fā)和應(yīng)用
3. 基因表達(dá)調(diào)控研究
工作經(jīng)歷：
2023至今 南方科技大學(xué) 研究副教授
2019 ~ 2023 南方科技大學(xué) 研究助理教授
2016 ~ 2019 南方科技大學(xué) 博士后
2015 ~ 2016 華大基因研究院 生物技術(shù)研究員
學(xué)習(xí)經(jīng)歷：
2007 ~ 2015 發(fā)育生物學(xué),，博士,，中國科學(xué)院遺傳與發(fā)育生物學(xué)研究所
2003 ~ 2007 生物技術(shù)，本科,，西北農(nóng)林科技大學(xué)
所獲榮譽(yù)：
2022 中國植物生理與植物分子生物學(xué)學(xué)會“優(yōu)秀女青年”
代表文章：
(*co-first authors, #corresponding author)
1. Zhang K.*, Zhao X.*, Zhao Y., Zhang z., Liu Z., Liu Z., Yu Y., Li Z., Ma Y., Dong Y., Pang X., Jin X., Li N., Liu B., Wendel J., Zhai J., Long Y.#, Wang T.#, Gong L.#. (2023) Cell type-specific cytonuclear coevolution in three allopolyploid plant species. PNAS. 120(40):e2310881120.
2. Mo W.*, Shu Y.*, Liu B. Long Y., Li T., Cao X., Deng X. #, Zhai J.# (2023) Single-molecule targeted accessibility and methylation sequencing of centromeres, telomeres and rDNAs in Arabidopsis. Nature Plants. 9, 1439–1450.
3. Liu Z.*, Yang J.*, Long Y.*, Zhang C.*, Wang D., Zhang X., Dong W., Zhao L., Liu C., Zhai J.#, and Wang E.# (2023) Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago. Nature Plants. https://doi.org/10.1038/s41477-023-01524-8
4. Liu Z.*, Kong X.*, Long Y.*, Liu S., Zhang H., Jia J., Cui W., Zhang Z., Song X., Qiu L., Zhai J.#, Yan Z.# (2023) Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule maturation. Nature Plants. https://doi.org/10.1038/s41477-023-01387-z.
5. Qin Y., Long Y., Zhai J.# (2022) Genome-wide characterization of nascent RNA processing in plants. Current Opinion in Plant Biology. 69:102294.
6. Jia J.*, Lu W.*, Liu B., Yu Y., Jin X., Shu Y., Long Y., Zhai J.# (2022) An atlas of plant full-length RNA reveals tissue-specific and evolutionarily-conserved regulation of poly(A) tail length. Nature Plants. 8(9):1118-1126.
7. Li Z.*, Long Y.*, Yu Y., Zhang F., Zhang H., Liu Z., Jia J., Mo W., Tian S. Z., Zheng M., Zhai J.# (2022) Pore-C simultaneously captures genome-wide multi-way chromatin interaction and associated DNA methylation status in Arabidopsis. Plant Biotechnology Journal. 20(6):1009-1011.
8. Yu Y.*, Zhang H.*, Long Y., Shu Y., Zhai J.# (2022) Plant Public RNA-seq Database: a comprehensive online database for expression analysis of ~45?000 plant public RNA-Seq libraries. Plant Biotechnology Journal. 20, 806-808.
9. Long Y.*, Jia J.*, Mo W., Jin X., Zhai J.# (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing. Nature Protocols. 16, 4355-4381.
10. Long Y.*, Liu Z.*, Jia J.*, Mo W., Fang L., Lu D., Liu B., Zhang H., Chen W., Zhai J.# (2021) FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants. Genome Biology. 22, 66.
11. Mo W.*, Liu B.*, Zhang H., Jin X., Lu D., Yu Y., Liu Y., Jia J., Long Y., Deng X., Cao X., Guo H., Zhai J.# (2021) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing. Genome Biology. 22(1):322.
12. Hu D.*, Yu Y.*, Wang C.*, Long Y., Liu Y., Feng L., Lu D., Liu B., Jia J., Xia R., Du J., Zhong X., Gong L., Wang K.#, Zhai J.# (2021) Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions. The Plant Cell. 33, 2950-2964.
13. Jia J.*, Ji R.*, Li Z., Yu Y., Nakano M., Long Y., Feng L., Qin C., Lu D., Zhan J., Xia R., Meyers BC, Liu B.#, Zhai J.# (2020) Soybean DICER-LIKE2 regulates seed coat color via production of primary 22-nucleotide small interfering RNAs from long inverted repeats. The Plant Cell. 32, 3662-3673.
14. Jia J.*, Long Y.*, Zhang H., Li Z., Liu Z., Zhao Y., Lu D., Jin X., Deng X., Xia R., Cao X., Zhai J.# (2020) Post-transcriptional splicing of nascent RNA contributes to widespread intron retention in plants. Nature Plants 6, 780-788.
15. Long Y.-P.*, Xie D.-J.*, Zhao Y.-Y., Shi D.-Q.#, and Yang W.-C.# (2019) BICELLULAR POLLEN 1 is a modulator of DNA replication and pollen development in Arabidopsis. New Phytologist. doi: 10.1111/nph.15610.
16. Zhang Y.*, Harris C.J.*, Liu Q.*, Liu W., Ausin I., Long Y., Xiao L., Feng L., Chen X., Xie Y., Chen X., Zhan L., Feng S., Li J., Wang H.#, Zhai J.# (2018) Large-scale comparative epigenomics reveals hierarchical regulation of non-CG methylation in Arabidopsis. PNAS. 115, E1069.
17. Fang H., Liu Z., Long Y., Liang Y., Jin Z., Zhang L., Liu D., Li H., Zhai J.# and Pei Y.# (2017) The Ca2+/calmodulin2- binding transcription factor TGA3 elevates LCD expression and H2S production to bolster Cr6+ tolerance in Arabidopsis. The Plant Journal. 91, 1038-1050.
18. Kuo H.Y., Jacobsen E.L., Long Y., Chen X., and Zhai J.# (2017) Characteristics and processing of Pol IV-dependent transcripts in Arabidopsis. Journal of Genetics and Genomics. 44, 3-6.
19. Wang, S.-Q., Shi, D.-Q., Long, Y.-P., Liu, J., and Yang, W.-C.# (2012) GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential for female gametogenesis and male competence in Arabidopsis. PloS one. 7, e33595.
專利：
1. 新型冠狀病毒樣顆粒及其制備方法和應(yīng)用,，翟繼先，龍艷萍,，賈津布,，溫艦宇，鹿東東,，申請?zhí)枺?02110333400.4,，發(fā)明專利
2. 一種帶編碼的凝膠微粒及其制備方法和應(yīng)用，翟繼先,，肖麗丹,，莫偉鵬，龍艷萍,，申請?zhí)枺?02010047034.1,，發(fā)明專利
3. 單細(xì)胞基因組測序用的DNA文庫的構(gòu)建方法，翟繼先,，龍艷萍,，肖麗丹，張飛,，鹿東東,，專利號：ZL 2019 1 1311804.2，授權(quán)日：2023-05-05發(fā)明專利